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Chinese Agricultural Science Bulletin

Abbreviation (ISO4): Chin Agric Sci Bull      Editor in chief: Yulong YIN

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Modification of the S1 nuclease Mutation Detection

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Online published: 2004-10-04

Abstract

Functional genomics require high-throughput mutation detection and S1 nuclease mutation detection is one of them, which has the advantages of low-cost and rapidness.But it is difficult to conquer the reaction and some perfectly matched sequence also will be degraded non-specificly. In this paper, the targeted mutagenesis promoter of chlorophyll a/b binding protein was exemplified, the procedure was streamlined and the cause of degrading duplex DNA by S1 nuclease non-specificly had also been elucidated.

Cite this article

Li Chunyu, Wang Tao , Liang Benguoand Li Guangchen . Modification of the S1 nuclease Mutation Detection[J]. Chinese Agricultural Science Bulletin, 2004 , 20(5) : 21 -21 . DOI: 10.11924/j.issn.1000-6850.040521

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