Home Journals Chinese Agricultural Science Bulletin
Chinese Agricultural Science Bulletin

Abbreviation (ISO4): Chin Agric Sci Bull      Editor in chief: Yulong YIN

About  /  Aim & scope  /  Editorial board  /  Indexed  /  Contact  / 
目次

Using Agrobacterium tumefaciens Obtained transgenetic Japonica plant

Expand

Online published: 2004-10-04

Abstract

The varieties are Kongyu131, Kenjiandao6 and Kenjiandao7 of Oryza stiva L subsup. Japonica. Agrobacterium tumefaciens infected maturate embryo of rice in order to induce callus.By this way, waxy gene can be transferred into accepted materials. After twice resistance selection, transgenetic seeding can be obtained in differentiation culture medium. Transgenetic plant is dyed by GUS and be identified by PCR, which can testify that exterior gene has been transferred into Japonica plant.

Cite this article

Liu Yongwei, Meng Qiaoxia, Dang Yongzhi, Meng Zhaohe, Li Chunguang, Liu Guoquan . Using Agrobacterium tumefaciens Obtained transgenetic Japonica plant[J]. Chinese Agricultural Science Bulletin, 2004 , 20(5) : 41 -41 . DOI: 10.11924/j.issn.1000-6850.040541

References

1易自力,曹守云,等.提高农杆菌转化水稻频率的研究.遗传学报,2001,28(4):352~358
2陈秀花,刘巧泉,等.根癌农杆菌介导转化籼稻影响因素的研究.江苏农业研究,2001,22(1):1~6
3翟文学,李晓兵,等.由农杆菌介导将白叶枯病抗性基因Xa21转入我国的5个水稻品种.中国科学,(C辑).2000,30(2):200~206
4刘巧泉, 张景六等.根癌农杆菌介导的水稻高效转化系统的建立.植物生理学报,1998,24(3):294~300
5Jefferson R A. Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol Biol Rep. 1987,5:387~405
6王关林,方宏筠.植物基因工程原理与技术.北京:科学出版社,1998,744
7周小云,饶力群.根癌土壤杆菌转化水稻研究进展.生物技术,2003,13(3):39~41
8尚玉磊,王琦.基因沉默与生物进化.生物技术,2003,13(4):41~44
Options
Outlines

/