we carried on cold pretreatment experiment during tomato anther culture to study the effect of cold pretreatment on microspores development during tomato anther culture in vitro. The results indicated that: Pathway B、A-V，and A-G, existed in androgenesis of tomato of which pathway B was predominent. Degeneration happened with some pollen; Cold pretreatment could delay degeneration of microspores to a certain extent and advance androgenesis; Cold pretreatment could also enhance the ratio of microspores participated in androgenesis, however pathway B increased greater than pathway A; In the early stage of culturing, cold pretreatment could increase the ratio of uninucleate microspores, which gave more chance for microspores to participate in androgenesis.
Zhou Guangdong, Wang Xiufeng, Xie Bing, Zhang de’an
. The effect of cold pretreatment on microspores development during in vitro culture oftomato anther[J]. Chinese Agricultural Science Bulletin, 2005
, 21(2)
: 192
-192
.
DOI: 10.11924/j.issn.1000-6850.0502192
1奚元龄,等编译.植物细胞培养手册.北京:中国农业出版社,1989.536~537
2Sharp W R, Dougall D K, Paddock E F(1971).来自烟草和番茄的未成熟花粉粒的单倍体愈伤组织和小植株.见:中国科学院北京植物研究所.单倍体育种集(第三集):北京:科学出版社,1977. 246~250
3Gresshoff P M, Doy C H.番茄单倍体的发育和分化，见: 中国科学院北京植物研究所.单倍体育种集(第二集).科学出版社,1973. 142~153
4高秀云,等.番茄花药离体获得植株.园艺学报,1980,7(4):27~41
5李文泽,胡含.不同预处理方法对大麦花药－花粉培养的影响.遗传,1995,17(5):5~7
6渠荣达,陈英.低温预处理提高水稻花粉愈伤组织诱导频率的作用.见: 陈英.植物细胞培养与遗传操作.长沙:湖南科学技术出版社,1992.108~117
7王心钗.植物显微技术.福州:福建教育出版社,1986.299
8刘萍,宋晓华,马惠萍,等.温度处理对春小麦花药愈伤组织诱导率影响初报.宁夏农学院学报,1996,17(1):152~56
9Genovesi A D,Collins G B.Invitro ptoduction of haploid plants of corn via anther culture[J].Crop Science,1982,22:1137~1144
10 原玉香,耿建峰,张晓伟,等.影响玉米花药培养效率的因素研究.华北农学院报,2001,16(3):12～16
11 曹晓燕,王喆之,赵银萍.毛刺槐花药陪及再生植株的获得.西北植物学报,2003,23(3):456~459
12 Huang B, Sunderland N. Temperature-stress pretreatment in barley anther culture.Ann Bot, 1982,49:77~88
13 Nitsch C, Norreel B. Effect d’um choc technique sur pepouvoir embrygnose du pollen de Datura innoxia cultive dansI’ anthere ou isole de I’anthere.C.R.Acad.Sci.Paris, 1973,276:303~306
14Hu T C, Kasha K J. A cytological study of pretreatments used to improve isolated microspore cultures of wheat (Triticum aestivum L.) cv. Chris,Ottawa,1999,42(3):432~441
15Pechan P M, Bartels D, Brown D C W, Schell J.Messenge-RNA and protein changes associated with induction of Brasstica microspore embryogenesis.Planta,1991,184:161~165
16许智宏,Sunderland N.大麦花粉在低温预处理及培养中DNA含量的显微光度测定.植物生理学报,1986,(12):140~147
17郭向荣,方红曼,李安生,等.甘露醇预处理方式、方法对大麦花粉植株再生的影响.农业生物技术学,1999,7(4):321~324
18黄斌.大麦花药培养中低温预处理对花药愈伤组织形成的影响.植物学报,1985,27(3):439~443
