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Chinese Agricultural Science Bulletin

Abbreviation (ISO4): Chin Agric Sci Bull      Editor in chief: Yulong YIN

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Chinese Agricultural Science Bulletin >

2005 , Vol. 21 >Issue 4: 48 - 48

DOI: https://doi.org/10.11924/j.issn.1000-6850.050448

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Construction of Recombinant Expression Plasmid pYELmleA of mleA Gene and Expression in Saccharomyces cerevisiae

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Online published: 2005-04-04

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Abstract

Malolactic enzyme is the function enzyme to turn L-malic acid into L-lactic acid during malolactic fermentation (MLF). In this paper, mleA gene encoding for malolactic enzyme from pLmleA, PGK1 promoter from plasmid pVC727-6 and ADH1 terminator from plasmid

Key words： Oenococcus oeni;Malo-lactic enzyme gene;Recombinant expression Plasmid;Saccharomyces cerevisiae

Cite this article

Liu Yanlin, Jiang Sixin, He Xiuping, Li Hua, Zhang Borun . Construction of Recombinant Expression Plasmid pYELmleA of mleA Gene and Expression in Saccharomyces cerevisiae[J]. Chinese Agricultural Science Bulletin, 2005 , 21(4) : 48 -48 . DOI: 10.11924/j.issn.1000-6850.050448

References

1李华.现代葡萄酒工艺学[M].西安:陕西人民出版社,2000. 2 Pascal R G, Denis D, Bernard D, et al. Handbook of Enology[M]. London: John Wiley & Sons Ltd. 2000 3 Labarre C, Guzzo J, Cavin J F, Divies C. Cloning and characterization of the genes encoding malolactic enzyme and the ma
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