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Chinese Agricultural Science Bulletin

Abbreviation (ISO4): Chin Agric Sci Bull      Editor in chief: Yulong YIN

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Identification and Differentiation into Cardiomyocytes of Goat Bone Marrow Mesenchymal Stem Cells in vitro

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Online published: 2005-05-04

Abstract

The Guanzhong dairy goats were used in our experiment to study the characteristics of their bone marrow mesenchymal stem cells (BM-MSCs), such as isolation, culture, and identification. The results indicate that there are excellent potentials for the goat mesenchymal-like stem cells derived from goat bone marrow to proliferate and to resist freezing and thawing repeatedly. The goat mesenchymal-like stem cells can express cellular surface markers CD34, CD44, CD105, CD106, CD 166, and TGF-α, but not CD14 and CD45. And after treated by 5-azacytidine in vitro, they express cellular surface marker of cardiac α-actin and Schiff’s stain positive.

Cite this article

Pang Quanhai,, Zhang Huiru, Hua Jinlian, Ma Yongjiang, Dou Zhongying . Identification and Differentiation into Cardiomyocytes of Goat Bone Marrow Mesenchymal Stem Cells in vitro[J]. Chinese Agricultural Science Bulletin, 2005 , 21(5) : 1 -1 . DOI: 10.11924/j.issn.1000-6850.050501

References

1Barry F P, et al. Mesenchymal stem cells: clinical applications and biological characterization. IJBCB, 2004,36:568~584
2Pittenger M F, et al. Multilineage potential of adult human mesenchymal stem cells. Science, 1999,284:143~147
3Barry F P, et al. Mesenchymal stem cells: clinical applications and biological characterization. IJBCB, 2004, 36:568~584
4Tropel P, et al. Isolation and characterisation of mesenchymal stem cells from adult mouse bone marrow. Experimental Cell Research, 2004, xx:1~12(SCIENCEDIRECT)
5Jiang Y, et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature,2002,418:41~49
6Li X S, et al. Modification of in situ cryopreservation of human bone marrow mesenchymal stem cells. J Exp Hematol,2003,11(5):530~533
7Meirelles L da S, et al. Murine marrow-derived mesenchymal stem cells: isolation, in vitro expansion, and characterization. British J Haematology, 2003, 123:702~711
8Hung S C, et al. Isolation and characterization of size-sieved stem cells from bone marrow. Stem Cells, 2002, 20:249~258
9Almeida-Porada G, et al. A large animal noninjury model for study of human stem cells plasticity. Blood Cells, Molecules, and Disease, 2004, 32:77~81
10 Lei J X,et al. Biological characteristics of long passaging rat mesenchymal stem cells. Chinese J of Pathophysiology, 2003,19(10):1325~1330
11 MacKenzie T C, et al. Human mesenchymal stem cells: Insights from a surrogate in vivo assay system. Cells Tissues Organs, 2002, 171:90~95
12 Barry F P. Biology and clinical applications of mesenchymal stem cells . Birth Defect Research (part C), 2003,69:250~256
13 Pang Q H, et al. Study and applications of human bone marrow mesenchymal stem cells. Chinese Medicine Research,2003,(14):5~6
14 MacKenzie T C, et al. Human mesenchymal stem cells persist, demonstrate site-specific multipotential differentiation, and are present in sites of wound healing and tissue regeneration after transplantation into fetal sheep. Blood Cells, Molecules, and Diseases, 2001,27(3):601~604
15 Bianco P, et al. Bone marrow stromal stem cells: Nature, biology, and potential applications. Stem Cells, 2001,19:180~192
16 Dominici M, et al. Bone marrow mesenchymal stem cells: biological properties and clinical applications. J Bio Regul and Homeost Agents, 2001,15:28~37
17 Meng X Q, et al. Bone marrow stem cells and kidney. Urology and nephrology FMS, 2004,24(2):258~261
18 Quintavalla J, et al. Fluorescently labeled mesenchymal stem cells (MSCs) maintain multilineage potential and can be detected following implantation into articular cartilage defects. Biomaterials,2002,23:109~119
19 Kruyt M.C, et al. Genetic marking with the ΔLNGFR-gene for tracing goat cells in bone tissue engineering. Journal of Orthopaedic Research,2004, 22: 697~702
20 Forrester J S, et al. Stem cell repair of infracted myocardium-A overview for clinician. Circulation,2003,108:1139~1145
21 Perry Tj?rvi E, et al. Bone marrow as a cell source for tissue engineering heart valves. Ann Thorac Surg, 2003,75:761~767
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