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Chinese Agricultural Science Bulletin

Abbreviation (ISO4): Chin Agric Sci Bull      Editor in chief: Yulong YIN

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The Study of Matic Cell Nucear Tranfer of Transgenin (t-PA) in Goat

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Online published: 2005-06-04

Abstract

The development of nuclear transfer embryos derived from fetal fibroblasts including t-PA gene and ovary granulose cells were compared. The cleavagetate (82.9%)and Morulae rate (55.9%)of ovary granulose cells weremore high than those of fetal fibroblasts(75.7%,48.7%). Blastocyst rate (19.7%) of ovary granulose cells was significantly higher than that of fetal fibroblasts (11.0%). The development of nuclear transfer embryos derived from fetal fibroblasts including t-PA gene in media DMEM/F12 including 0.5%FBS for starvation and including 10%FBS were compared. The cleavagetate(75.6%) of nuclear transfer embryos in media DMEM/F12 adding 0.5%FBS is not significantly defferenter than that (75.8%)in media DMEM/F12 adding10%FBS. Morular rate (46.5%)and blastocyst rate (11.0%)in media DMEM/F12 including 0.5%FBS were higher than those (39.3%, 9.0%)in media DMEM/F12 adding10%FBS (P>0.05). The blastocyst rate(9.1%) nuclear transfer embryos in media SOF adding 10% bFF was higher that(4.2% )10% FBS. The blastocyst rate(11.2%) nuclear transfer embryos in media SOF adding 10% bFF was higher that(5.5% )10% FBS. SOF was better than CR1 for the early development in nuclear transfer embryos.

Cite this article

Liu Fengjun, Wang Yongsheng, Zhang Yong . The Study of Matic Cell Nucear Tranfer of Transgenin (t-PA) in Goat[J]. Chinese Agricultural Science Bulletin, 2005 , 21(6) : 1 -1 . DOI: 10.11924/j.issn.1000-6850.050601

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