The development of nuclear transfer embryos derived from fetal fibroblasts including t-PA gene and ovary granulose cells were compared. The cleavagetate （82.9%）and Morulae rate （55.9%）of ovary granulose cells weremore high than those of fetal fibroblasts(75.7%,48.7%). Blastocyst rate (19.7%) of ovary granulose cells was significantly higher than that of fetal fibroblasts (11.0%). The development of nuclear transfer embryos derived from fetal fibroblasts including t-PA gene in media DMEM/F12 including 0.5%FBS for starvation and including 10%FBS were compared. The cleavagetate(75.6%) of nuclear transfer embryos in media DMEM/F12 adding 0.5%FBS is not significantly defferenter than that (75.8%)in media DMEM/F12 adding10%FBS. Morular rate (46.5%)and blastocyst rate (11.0%)in media DMEM/F12 including 0.5%FBS were higher than those (39.3%, 9.0%)in media DMEM/F12 adding10%FBS （P＞0.05）. The blastocyst rate(9.1%) nuclear transfer embryos in media SOF adding 10% bFF was higher that(4.2% )10% FBS. The blastocyst rate(11.2%) nuclear transfer embryos in media SOF adding 10% bFF was higher that(5.5% )10% FBS. SOF was better than CR1 for the early development in nuclear transfer embryos.
Liu Fengjun, Wang Yongsheng, Zhang Yong
. The Study of Matic Cell Nucear Tranfer of Transgenin (t-PA) in Goat[J]. Chinese Agricultural Science Bulletin, 2005
, 21(6)
: 1
-1
.
DOI: 10.11924/j.issn.1000-6850.050601
1连正兴,张磊,刘兆良,等.整合人mAAT基因转基因山羊的获得[J].遗报,2001,28(8):716~721
2Wilmut I, Schnieke A E, Mc Whir J, Kind A J, Campbell K H S. Viable offsoing derived fromfetal and dult mammalian cells [J]. Nature,1997,385:810~813
3Schnieke A E, Kind A J, Ritchie W A, et al. Human factorIX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblaatsp[J]. Science,1997,278:2130~2133
4翟少伟,谷艳钗.转基因动物的研究进展和前景展望[J].当代畜牧,2000,(6):1~3
5沈子龙,廖建民,徐寒梅.转基因动物技术与转基因动物制药[J].中国药科大学学报,2002,33(2):81~86
6Baguisi A, Behboodi E, Melian DT, et al. Production of goats by somatic cell nuclear transfer. Nat Biotechnol. 1999,17(5):456~461
7Cibelli JB, Stice S L, Golueke P J, et al. Cloned transgenic calves produced from nonquiescent fetal fibroblast. Science,1998,280(5):1256~1258
8Onishi A, Iwanmoto M, Akita T, et al. Pig cloning by microinjection of fetal fibroblast nuclei.Science. 2000,289:1188~1190
9Chenog H T, Ikeda K, Martinez-Diaz M A, et al. Development of reconstituted pig embryos by nuclear transfer of cultured cumulus cells. Fertil. Dev.,2000,12:15~20
10 Kato Y, Tani T, Sotomaru Y, et al. Eight calves cloned from somatic cells of a single adult. Science. 1998,282:2095~2098
11 Kubota M, Yamakuchi H, Todorochi J, et al. Six cloned calves produced from adult fibroblast cells after long-term culture. Proc. Natl. Acad. Sci. USA.,2000,97:990~995
12 侯健,陈学进,安晓荣,等.哺乳动物体细胞核移植研究进展应用前景,生物技术通报,1999,(5):16~19
13 杨素芳,石德顺,韩杰,等.供体核种类对水牛核移植效果的影响.广西农业生物科学,2004,23(3):234~237
14 akayama T, Perry A C F, Zoccotti M, et al. Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature,1998,394(7):369~374
15 Collas P, Balise J J, Robl J M. Influence of cell cycle stage of the donor nucleus on development of nuclear transplant rabbit embryos. Biol Reprod. 1992,46:492~500
16 张治芬.人血清及卵泡液中巨噬细胞集落刺激因子水平的测定[J].现代妇产科进展,2001,10(5):337~339
17 Martin K L, Barlow D H and Sargent I L. Heparin-binding epidermal growth factor significantly improves human blastocyst development and hetching in serum free medium. Human Reproduction, 1998,13:1645
18 Hemmings R, Lachapelle M H, Falcone T, Miron P, Ward L and Guyda H. Effect of follicular fluid supple mentation on the In Vitro development of human preembryos. Fertility and Sterilitl,1994,62:1018~1021
19 Colver R M, Howe A M, McDonough P G and Boldt J. Influence of growth factors in defined culture medium on in vitro development of mouse embryos. Fertility and Sterilitl,1991,55:194~195
