mRNA differential display reverse transcription polymerase chain reaction（DDRT-PCR）is an effective technology in isolation and cloning genes in molecular biology. The primary principle, superiorities, shortages, andtechnological improvements were reviewed, and this paper mainly focuses on the application of the technology in the isolation of rice (Oryza sativa L.) genes.
Cheng Yanjun, ，Gao Dongying, Liu Aimin，Tao Lan
. The Application of mRNA Differential Display in Isolation of Genes in Rice(Oryza sativa L.)[J]. Chinese Agricultural Science Bulletin, 2004
, 20(1)
: 29
-29
.
DOI: 10.11924/j.issn.1000-6850.040129
1 Liang Peng,Arthur B Pardee. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science,1992,257(14):967～971
2 黄旭,龚蓁蓁,徐竹筠等.mRNA差异显示技术分离水稻抗稻瘟病相关cDNA克隆.江苏农业学报,2000,16(2):124～126
3 董海涛,董继新,何祖华等.水稻受稻瘟病菌诱导的转座类似蛋白新基因RIM2的分离与鉴定.农业生物技术学报.2001,9(2)：113～118
4 Dong Haitao,Wu Yuliang,Cheng Zhiqiang,He Zuhua,Li Debao.Molecular Cloning of Differentially Expressed Gene with Kinase Motif Induced by Xanthomonas oryzae pv.oryzae.Journal of Zhejiang Agricultural Umiversity,1998,24(5):548～552
5 Qin Qing-ming,ZHANG Quan,ZHAO Wen-sheng,et al.Identification of a lectin gene induced in rice in response to magnaporthe grisea infection.Acta Botanica Sinica,2003,45(1):76～81
6 Cheng Zhiqiang,Dong Haitao,Wu Liangyu,et al.Cloning of resistance-related gene fragment induced by xanthomonas oryzae pv.oryzae.Journal of Agricultural Biotechnology,2000,8(1):
7 张弛,陈受宜.[J].中国科学,1995,25(8):840～847
8 于福同,张爱民,陈受宜等.遗传学报,2001,28(2):144～151
9 丁秀英,张军,崔霞等.低温胁迫下IPT诱导水稻幼苗根中的mRNA差异显示分析.作物学报,2001,27(6):935～940
10 张立平,吴平,祝金明等.利用DD-PCR技术分析水稻铝诱导基因的表达差异.中国农业科学,1997,30(5):71～74
11 程宁辉,高燕萍,杨金水等.水稻杂种一代与亲本幼苗基因表达差异的分析.植物学报,1997,39(4)：379～382
12 顾克余,翟虎渠等.水稻杂种一代及其亲本分蘖期根系基因的差异表达.南京农业大学学报,2000,23(1)：1～4
13 凌杏元,周培疆,朱英国等.水稻红莲型细胞质雄性不育系与保持系mtRNA差异显示和差异片段的分析,植物学报,2000,42(3)：284～288
14 Wang Xue de,Zhu Ying guo.mRNA differential display between sterile and fertile anther of rice and analysis of cDNA differential fragments.SCIENCE IN CHINA(Series C).Vol.41No.2:217～224
15 关和新,景润春,朱英国等.[J].中国水稻科学,1998,12(4):215～218
16 康健,陈凡,吴乃虎.[J].科学通报,1998,43(19):2078～2082
17 Akaki H,Sakamoto M,Shinjyo C,et al.A unique sequence located downstream from the rice mitochondrial atp6 may cause male sterility.Curr Genet,1994,25:52～58
18 王光清,杨金水,程宁辉等.[J].实验生物学报,1997,30(1)：21～26
19 Knaap E van der,Knende H Identification of a gibberellin-induced gene in deepwater rice using differential display of mRNA[J].Plant Mol Biol,1995,28:589～592.
20 李竑,沈思师,许智宏等.水稻eIF3大亚基eIF3a编码基因的克隆及其表达模式分析.实验生物学报,2003,36(1):54～60
21 Esther V,Sandrine J,Hans K,et al.[J].Proc Natl Acad Sci USA,1997,94:9979～9983
22 Tseng T C,Tsai T H,Lee H T,et al.[J].Gene,1995,161:179～182
23 Guimarase M J,Frank L,Mclanahan T,et al.[J].Nucleic Acids Research,1995,23(10):1832～1833
24 Liang,P.et al.1993.Distribution and cloning of eukaryotic mRNA by means of differential display :refinemeng and optimization. Nucleic Acid Research21:3269～3275
25 Lauren S,Stephen J,Timothy C,B.et al.1995.Overvcoming limitations of the mRNA differential display technique. Nucleic Acids Research 23(22):4738～4739
26 关和新,凌杏元,朱英国等. 琼脂糖电泳展示mRNA差异方法的探讨.中国水稻科学,1998,12(3):177～180
