A high regeneration frequency system of watermelon was set up by using the watermelon cultivated species- ZhongYuYiHao. Results indicated that MS+6-BA1.0+IBA0.5 was the most suitable medium for cotyledon and MS+6-BA1.0+IBA0.1 for stem apex；the strectched shoot rooted easily in MS+IAA0.2；low temperature and high humidity may be helpful for the survival of test-tube seedlings.
ZHANG Zhizhong，WU Jinghua，LU Liuxin
. A Preliminary Study on Establishment High Regeneration Frequency System of Watermelon[J]. Chinese Agricultural Science Bulletin, 2004
, 20(2)
: 151
-151
.
DOI: 10.11924/j.issn.1000-6850.0402151
1Zhang X，Prodes B B．RAPD molecular marker in watermelon．Hortscience，1993，28(5)：223
2Lee H K，Seo Y K，Rhee W Y．Genotypic effect of watermelon (Citrullus lanatus Thunb．) on organogenesis from shoot
tip culture of seedlings．Korean Journal of Plant Tissue Culture，1994，21(4)：239-246．
3Sanchez Donaire A，Guerra Sanz J M，Encina C L．In vitro watermelon genotype screening by adventitious shoot
induction from juvenile and immature cotyledons．Cucurbit-Genetics-Cooperative．2000，23：49-50．
4Hao Lixing，Wang Huaiming．A study on building up the regeneration system of watermelon．Acta Agriculturae Boreali-Sinia，1998，13(3)：112-115．
5Szalai J．The micropropagation of watermelon．Kerteszeti Tudomany，1995，27(4)： 111-113．
6Reng Chunmei，Dong Yanyu，Hong Yahui，et al．Tissue culture of watermelon．Journal of Hunan Agriculture University，2000，26(1)：50-53．
7Song Daojun，Cheng Ruolei，Ying Ruochun et al．A Preliminary Study on tissue culture and high regeneration frequency system of watermelon．Chinese Watermelon and Melon，2000，4：8-11.
